rpoB-PCR amplified gene and temporal temperature gradient gel electrophoresis: a rapid tool to analyse bacterial strains representative of cold-smoked salmon microflora
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چکیده
منابع مشابه
rpoB-PCR amplified gene and temporal temperature gradient gel electrophoresis: a rapid tool to analyse bacterial strains representative of cold-smoked salmon microflora.
AIM To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains. METHODS AND RESULTS DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpo...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2004
ISSN: 0266-8254,1365-2673
DOI: 10.1111/j.1472-765x.2003.01463.x